#IMAGE PRO PLUS USER SETTINGS MANUAL#
#IMAGE PRO PLUS USER SETTINGS FULL#
#IMAGE PRO PLUS USER SETTINGS SOFTWARE#
#IMAGE PRO PLUS USER SETTINGS PC#
#IMAGE PRO PLUS USER SETTINGS PLUS#

Meanwhile, drawing the adipocyte boundaries manually by IPP and ImageJ is accurate but time-consuming and intractable to large-scale investigations. However, because adipocytes are membrane-stained, and clear contrast or threshold is difficult to be set by these tools, the automatic counting of adipocyte sizes on adipose tissue slides may result in severe measurement errors. Area of interest (AOI) and measurement threshold of adipose tissue slides can be set to screen out objects that meet the requirements automatically ( 11, 12).

#IMAGE PRO PLUS USER SETTINGS PLUS#

ImageJ and Image-Pro Plus (IPP) are both the open-software platform image processing tools for scientific image analysis and have been widely used in life sciences ( 9, 10).

#IMAGE PRO PLUS USER SETTINGS SOFTWARE#

Therefore, quantification of the number and size of adipocytes is essential for assessing the severity of obesity and providing the reference for the choice of treatment ( 5).ДЄs technology advances, image processing software have been developed for the measurement of adipocytes ( 6вЂ“ 8). When the capacity of adipocytes cannot meet the demand for lipid storage, excess lipids will be ectopically deposited in the muscles, liver, and pancreas, causing metabolic abnormalities, such as insulin resistance ( 3, 4). Obesity results from energy intake in excess of energy expenditure, and surplus energy is stored in adipocytes as a form of triglycerides, which results in adipocyte hyperplasia and hypertrophy ( 1, 2). Overall, we developed a combination method to measure adipocyte sizes accurately and efficiently, which may be helpful for experimental process in laboratory and also for clinic diagnosis. Besides, the recognition effect of monochrome segmentation image is better than that of color segmentation image.

#IMAGE PRO PLUS USER SETTINGS MANUAL#

We found that the adipocyte sizes quantified by the tool combination were comparable as manual measurement, whereas the combined methods were more efficient.

In the present study, we introduced the protocol of combined usage of these tools and illustrated the process with histological staining slides from adipose tissues of lean and obese mice. Although commercially available adipocyte counting tools, including AdipoCount, Image-Pro Plus, and ImageJ were helpful, limitations still exist in accuracy and time consuming. Manual measurement is accurate but time-consuming.

After histological staining, it is critical to accurately measure the number and size of adipocytes for assessing the severity of obesity in a timely fashion. One of the major characteristics of obesity is fat accumulation, including hyperplasia (increase in number) and hypertrophy (increase in size). In recent decades, the prevalence of obesity has been rising.

3Key Laboratory of Adolescent Health Assessment and Exercise Intervention of Ministry of Education, College of Physical Education and Health, East China Normal University, Shanghai, China.

2Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, Shanghai, China.

1Department of Endocrine and Metabolic Diseases, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.

If youвЂ™ve edited a ProRAW photo in the Photos app on your Mac,В andВ you want toВ use your Mac to share the original ProRAW file only, select the photo in the Photos app, then choose File > Export > ExportВ Unmodified Original.Yepeng Hu 1вЂ , Jian Yu 2вЂ , Xiangdi Cui 2, Zhe Zhang 2, Qianqian Li 1, Wenxiu Guo 2, Cheng Zhao 2, Xin Chen 2, Meiyao Meng 2, Yu Li 2, Mingwei Guo 2, Jin Qiu 2, Fei Shen 3, Dongmei Wang 2, Xinran Ma 1,2, Lingyan Xu 1,2*, Feixia Shen 1* and Xuejiang Gu 1*.

#IMAGE PRO PLUS USER SETTINGS PC#

If you edit a ProRAW photo and want to share both the original ProRAW file and the edited JPG file, use Image Capture on Mac or the Windows Photos app on a PC to import the files.

The Mail app automatically converts the ProRAW file to a smaller JPG file, which you can then send with email.

To email a ProRAW photo, create an email in the Mail app on your iPhone, then add the photo to the email.

To share an edited ProRAW photo with someone else, edit the photo in the Photos app on your iPhone, then AirDrop, email, or send with Messages.В A JPG file that includes your edits is shared.

To share the original ProRAW file from the Photos app on your iPhone, tap Share, tap Duplicate, tap Edit, tap Revert, then share the photo using AirDrop.

To transfer the original ProRAW file from your iPhone to another iPhone, iPad, or your Mac, use AirDrop.В If you edited the photo in the Photos app and then you share it, you'll share a JPG file.

#IMAGE PRO PLUS USER SETTINGS FULL#

Use iCloud Photos to access the original ProRAW file in full resolution on all of your devices.

Here are the best ways to share your ProRAW photos:

Image pro plus user settings